Human TXNRD1 (Thioredoxin reductase 1, cytoplasmic) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	TXNRD1
Gene ID:	7296
Uniprot ID:	TRXR1_HUMAN
Specificity:	This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Isoform 1: Expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4: Widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
Post Translational Modifications	Isoform 5: The N-terminus is blocked. ISGylated.
Function	Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form. Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2). Isoform 1: Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4: Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2. Isoform 5: Enhances the transcriptional activity of the estrogen receptor ESR2 only. Mediates cell death induced by a combination of interferon-beta and retinoic acid.
Protein Name	Thioredoxin Reductase 1 - Cytoplasmic Tr Gene Associated With Retinoic And Interferon-Induced Mortality 12 Protein Grim-12 Gene Associated With Retinoic And Ifn-Induced Mortality 12 Protein Km-102-Derived Reductase-Like Factor Peroxidase Txnrd1 Thioredoxin Reductase Tr1
Database Links	Reactome: R-HSA-1989781 Reactome: R-HSA-2408550 Reactome: R-HSA-3299685 Reactome: R-HSA-499943 Reactome: R-HSA-5263617 Reactome: R-HSA-5336415 Reactome: R-HSA-5628897 Reactome: R-HSA-9818027
Cellular Localisation	Isoform 1: Cytoplasm Isoform 4: Cytoplasm Nucleus Isoform 5: Cytoplasm
Alternative CLIA Names	Thioredoxin Reductase 1 - Cytoplasmic CLIA kit Tr CLIA kit Gene Associated With Retinoic And Interferon-Induced Mortality 12 Protein CLIA kit Grim-12 CLIA kit Gene Associated With Retinoic And Ifn-Induced Mortality 12 Protein CLIA kit Km-102-Derived Reductase-Like Factor CLIA kit Peroxidase Txnrd1 CLIA kit Thioredoxin Reductase Tr1 CLIA kit TXNRD1 CLIA kit GRIM12 CLIA kit KDRF CLIA kit
Specificity	This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-104	96
EDTA plasma(n=8)	89-102	96
Cell culture media(n=8)	93-106	100

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	94.97	300.95	929.28	99.80	279.57	841.33
Standard deviation	11.90	35.78	77.32	9.01	21.47	85.56
CV (%)	12.53	11.89	8.32	9.03	7.68	10.17

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance