RayBio® Human IL-1 Ra (IL-1 F3) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
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Human IL-1 RA ELISA Kit
Human IL-1 RA ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 100 pg/ml Need more sensitivity? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.1 ng/ml - 30 ng/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P18510 |
| Gene Id | 3557 |
| Gene Symbols | IL1RN, IL1RA, IL1F3 |
| Protein Name / Synonyms | Interleukin-1 receptor antagonist protein (IL-1RN) (IL-1ra) (IRAP) (ICIL-1RA) (IL1 inhibitor) (Anakinra) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Immunological markers of drug resistant epilepsy_x000D_ and its response to immunomodulatory therapy with ACTH_x000D_ in children
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Folia Neuropathologica
Does intestinal epithelial integrity status in response to high-protein dairy milk beverage with or without progressive resistance training impact systemic inflammatory responses in an active aging population?
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Plos One
Resistance Training Improves Sleep and Anti-Inflammatory Parameters in Sarcopenic Older Adults: A Randomized Controlled Trial
- Species:
- Human
- Sample Type:
- blood
- Year:
- 2022
- Journal Name:
- International Journal of Environmental Research and Public Health
Interleukin 1 receptor antagonist (IL1Ra) VNTR polymorphism influences circulatory IL1Ra levels and development of SLE in South Indian Tamils
- Species:
- Human
- Sample Type:
- Serum (Indian SLE Patients)
- Year:
- 2016
- Journal Name:
- Indian Journal of Rheumatology
Association of fetuin A, adiponectin, interleukin 10 and total antioxidant capacity with IVF outcomes
- Species:
- Human
- Sample Type:
- Serum (IVF patients)
- Year:
- 2014
- Journal Name:
- Iranian Journal of Reproductive Medicine
Serum sTNFR1, IL6, and the Development of Fatigue in Patients with Gastrointestinal Cancer Undergoing Chemoradiation Therapy
- Species:
- Human
- Sample Type:
- Serum (Colorectal or esphageal cancer patients treated with concurrent chemoradiation therapy)
- Year:
- 2012
- Journal Name:
- Brain, Behavior and Immunity
Bone morphogenetic protein 7 polarizes THP-1 cells into M2 macrophages
- Species:
- Human
- Sample Type:
- Conditioned Media (THP-1 cells polarized to M2 macrophages treated with BMP7)
- Year:
- 2012
- Journal Name:
- Canadian Journal of Physiology and Pharmacology.
- PubMed ID:
- 22720873
Steroids induce a disequilibrium of secreted interleukin-1 receptor antagonist and interleukin-1 synthesis by human neutrophils.
- Species:
- Human
- Sample Type:
- Conditioned Media (Neutrophils ); Plasma
- Year:
- 2011
- PubMed ID:
- 20650986
Bacterial vaginosis: risk factors and vaginal lavage cytokines IL-1b, IL-1ra.
- Species:
- Human
- Sample Type:
- Conditioned Media (vaginal lavage samples)
- Year:
- 2011