RayBio® G-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.
Human Cytokine Array G3
Human Cytokine Array G3
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 42 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
ENA-78 (CXCL5)
| GCSF
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
|
I-309 (TCA-3/CCL1)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-3
|
IL-4
| IL-5
| IL-6
| IL-7
| IL-8 (CXCL8)
|
IL-10
| IL-12 p40/p70
| IL-13
| IL-15
| IFN-gamma
|
MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| M-CSF
| MDC (CCL22)
|
MIG (CXCL9)
| MIP-1 delta (CCL15)
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
| EGF
|
IGF-1
| Angiogenin
| Oncostatin M
| Thrombopoietin (TPO)
| VEGF-A
|
PDGF-BB
| Leptin
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array G3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-CYT-G3-4.gal)
GAL (GAL-AAH-CYT-G3-8.gal)
Excel (AAH-CYT-G3_Analysis_Tool.xls)
citations
CD8+ T cell loss induces autoinflammation in inborn errors of cell death
- Species:
- Human
- Sample Type:
- Serum (patient before/after fever onset)
- Year:
- 2026
- Journal Name:
- Nature Communications
- PubMed ID:
- 41813671
Tp53 determines the spatial dynamics of M1/M2 tumor-associated macrophages and M1-driven tumoricidal effects
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (M0, M1 macrophage conditioned media)
- Year:
- 2025
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 39843434
Therapeutic effects of conditioned medium of immortalized dental pulp stem cells from human exfoliated deciduous teeth against experimental autoimmune neuritis
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned medium SHED-CM)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 41188337
Low Baseline CXCL9 Predicts Early Progressive Disease in Unresectable HCC with Atezolizumab Plus Bevacizumab Treatment
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Liver cancer
Regulation of CCL2 by EZH2 affects tumor-associated macrophages polarization and infiltration in breast cancer
- Species:
- Human
- Sample Type:
- THP-1 cell
- Year:
- 2022
- Journal Name:
- Cell Death & Disease
Original research: Generation and proof-of-concept for allogeneic CD123 CAR-Delta One T (DOT) cells in acute myeloid leukemia
- Species:
- Human
- Sample Type:
- Delta One T cells (DOTs)
- Year:
- 2022
- Journal Name:
- Journal for immunotherapy of cancer
Conditioned media of stem cells from human exfoliated deciduous teeth contain factors related to extracellular matrix organization and promotes corneal epithelial wound healing
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (conditioned media from SHED, other MSCs)
- Year:
- 2025
- Journal Name:
- Regenerative Therapy
- PubMed ID:
- 40170802
Novel plasma cytokines identified and validated in children during lead exposure according to the new updated BLRV
- Species:
- Human
- Sample Type:
- Plasma (children with elevated vs low blood lead)
- Year:
- 2024
- Journal Name:
- Scientific Reports
- PubMed ID:
- 39639084
circFLNA promotes intestinal injury during abdominal sepsis through Fas-mediated apoptosis pathway by sponging miR-766-3p
- Species:
- Human
- Sample Type:
- HIEC-6 cell
- Year:
- 2023
- Journal Name:
- Inflammation Research
Cytokine profile of cerebrospinal fluid in pediatric patients with metastatic medulloblastoma
- Species:
- Human
- Sample Type:
- Cerebrospinal Fluid (pediatric medulloblastoma patients vs controls)
- Year:
- 2024
- Journal Name:
- Heliyon
- PubMed ID:
- 39524698