Affinity resins for antibodies
The basis for purification of IgG, IgG fragments and subclasses is the high affinity of protein A (cell wall protein derived from Staphylococcus aureus ) and protein G( derived from groups C and G Streptococci )for the Fc region of polyclonal and monoclonal IgG-type antibodies. Protein A can be used to isolate monoclonal and polyclonal IgG from ascites, serum, and tissue culture and bioreactor supernatants. Protein A purification is recommended for human (except IgG3; mouse IgG1 may bind only weakly), rabbit, guinea pig, and pig antibodies. Addition of the antibody to a protein A– Sepharose column at pH 8.0, followed by elution at a lower pH.
Protein G has a binding profile opposite to that of protein A with respect to pH: antibodies bind better at a low pH and badly at high pH. However, some antibodies (mouse IgG1, and rabbit and human antibodies) do remain bound to protein G at high pH (8 to 10), so it is best to bind the antibody at pH 5 and elute at pH 2.8. This method is useful for mouse IgG1, rat (most subclasses bind weakly although IgG2b may not), monkey, rabbit, cow, goat, horse, and sheep antibodies.
Frequently Asked Questions (FAQ)
Where can I find antibody products on the CliniSciences website?
antibody products are available in this section and can be sorted by product families or sub-categories, such as Protein A Affinity resins, Protein G Affinity resins. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the antibody category?
The antibody category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Protein G Affinity resins, Protein A Affinity resins sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a antibody product?
To find a antibody product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does CliniSciences stock several product codes in the antibody category?
Yes, CliniSciences distributes several product references in the antibody category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for antibody products?
Yes, you can request a quote for antibody products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a antibody product is available to order?
The availability of a antibody product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.
What should I do if I cannot find the antibody product I am looking for?
If the antibody product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all antibody products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.