Protein A Affinity resins

Protein A Affinity resins

Protein A is a cell wall protein from Staphylococcus aureus with a molecular weight between 35-50 kDa and shows high specificity for the Fc region of immunoglobulin molecules of many mammalian species. It is encoded by the spa gene, and its regulation is carried out at the level of the DNA topology, as a function of cellular osmolarity, by a two-component system called ArlS-ArlR. The quality of the resin (Protein A Agarose or equivalent) and stability of the immobilisation is important to avoid leakage of Protein A during the elution procedure.
Protein A affinity chromatography is a rapid one-step purification, which removes most nonIgG contaminants and can achieve purities close to homogeneity. It is particularly useful for purifications of tissue culture supernatant, where 10-100 fold concentrations can be achieved.

 Ni-NTA-coordination
Specifications:
  • Resin: Protein A Agarose F
  • Base Matrix: Agarose
  • Protein A capacity (hIgG): 30 mg (per 1 ml resin)

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Cat#
Description
Cond.
Price Bef. VAT
NB-45-00036-5
 5ml 
NB-45-00036-25
 25ml 
NB-45-00036-1
 1ml 
NB-45-00036-100
 100ml 
DP301-01
 5ml 
orb1742840-25ml
 25ml 
orb1742840-250ml
 250ml 
orb1742840-100ml
 100ml 
orb1742840-5ml
 5ml 
abx098111-5ml
 5ml 
BSP096-5
 2.5ml 
BSP095-8
 5ml 
00830-02-01
 1mL 
00830-02-02
 2mL 
00830-02-05
 5mL 
abx291361-25ml
 25ml 
abx291361-500ml
 500ml 
abx291361-5ml
 5ml 
PA09-500
 500ml 
PA09-100
 100ml