Horseradish peroxidase (HRP)-conjugated anti-guinea pig antibodies are enzymatic immunoreagents designed for the sensitive detection of guinea pig antigens in colorimetric and chemiluminescent immunoassays. HRP catalyzes substrate conversion to generate amplified detection signals, making these conjugates indispensable for Western blotting, ELISA, and immunohistochemistry (IHC).

Formats and Specificity

HRP-conjugated antibodies are available as whole IgG, Fc fragment-specific, or F(ab')₂ fragments produced in goat or rabbit hosts. Whole IgG antibodies recognize both heavy and light chains (H+L), whereas Fc-specific conjugates selectively bind the Fc region of guinea pig IgG. Antibody specificity is validated by immunoelectrophoresis and ELISA, with no detectable reactivity toward non-immunoglobulin serum proteins. Cross-adsorbed preparations further reduce off-target binding to immunoglobulins from other species.

Applications and Recommended Dilutions

• Immunohistochemistry (IHC) & Immunocytochemistry (ICC): Recommended dilution of 1:500–1:5,000 for sensitive tissue and cell staining.
• Western blotting & ELISA: Typical dilutions range from 1:5,000–1:100,000 with chromogenic substrates and 1:10,000–1:200,000 when using enhanced chemiluminescence (ECL).
• Optimized formats: F(ab')₂ fragments are preferred for live-cell applications, minimizing Fc receptor interactions and reducing nonspecific binding.

Storage Recommendations

These conjugates are supplied either as lyophilized preparations or liquid formulations in PBS/BSA. Store freeze-dried products at 2–8 °C and reconstitute with distilled water (dH₂O) before use. For long-term preservation, aliquot the reconstituted antibody and store at −20 °C in 50% glycerol. Sodium azide should not be added because it irreversibly inhibits HRP enzymatic activity.