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Mouse Monoclonal Anti-MUC5AC (Mucin 5AC / Gastric Mucin) [Clone CLH2]
Cat# NB-36-00535-P0
Size : 20ug
Brand : Neo Biotech
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Phone : +1 850 650 7790
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Printable Datasheet
MUC5AC (Mucin 5AC / Gastric Mucin) Antibody [CLH2]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Mouse Monoclonal Antibody (CLH2). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, University of Porto, Portugal.
SDS-PAGE Analysis of Purified MUC5AC Mouse Monoclonal Antibody (CLH2). Confirmation of Integrity and Purity of Antibody
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
Mucin 5AC glycoprotein (MUC5AC) is a HMW glycoprotein belonging to the superfamily of mucins. Mucins are produced by epithelial cells and can be divided into two families; secretory mucins and membrane bound mucins. MUC5AC is a mucus-forming, secreted mucin that is found in normal gastric and tracheo-bronchial mucosa, but absent from normal colon. MUC5AC expression is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. The antibody recognizes the sequence TTSTTSAP within the tandem repeat of MUC5AC. It further recognizes glycosylated as well as unglycosylated MUC5AC.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
MUC5AC
Positive Control
Human colon or stomach (IHC).
Immunogen
A synthetic peptide of human MUC5AC tandem repeat
Alternate Names
Mucin-5AC, Gastric mucin, Major airway glycoprotein, Mucin-5 subtype AC, tracheobronchial, Tracheobronchial mucin, Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)
Functions
- Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).
Key References
- Reis CA, David L, Nielsen PA, Clausen H, Mirgorodskaya K, Roepstorff P, et al. Immunohistochemical study of MUC5AC expression in human gastric carcinomas using a novel monoclonal antibody. Int J Cancer 1997;74:112-
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/16554811/
- https://pubmed.ncbi.nlm.nih.gov/11535137/
- https://pubmed.ncbi.nlm.nih.gov/9506983/
- https://pubmed.ncbi.nlm.nih.gov/8948439/
- https://pubmed.ncbi.nlm.nih.gov/2656675/
- https://pubmed.ncbi.nlm.nih.gov/9620876/
- https://pubmed.ncbi.nlm.nih.gov/7513696/
- https://pubmed.ncbi.nlm.nih.gov/7775418/
- https://pubmed.ncbi.nlm.nih.gov/16787389/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/17974005/
- https://pubmed.ncbi.nlm.nih.gov/11445551/
- https://pubmed.ncbi.nlm.nih.gov/14535999/
- https://pubmed.ncbi.nlm.nih.gov/14718370/
- https://pubmed.ncbi.nlm.nih.gov/22186971/
- https://pubmed.ncbi.nlm.nih.gov/25939779/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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