Anti-3 X Flag Antibody
Anti-3 X Flag Antibody
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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Species | Human |
| Host Species | Rabbit |
| Protein Name / Synonyms | 3 X Flag |
| Clonality | Polyclonal |
| Immunogen | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen 3 X Flag. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Immunogen Sequence | CDYKDHDGDYKDHDIDYKDDDDK |
| Recommended Applications | ELISA (recommended work dilution= 1:32, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in 1X PBS, aliquot using ddH2O. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
3-Flag peptide has found widespread use as a mild purification reagent for Flag-epitope tagged recombinant proteins. Ability to bind heavy metals was a significant finding. 3x FLAG epitope is hydrophilic, 22-amino-acids long and can detect up to 10 fmol of expressed fusion protein. FLAG-tag can be removed by treatment with enterokinase, which is specific for the five C-terminal amino acids of the peptide sequence5.
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.