Library quantifcation kits for Illumina®
Accurate quantitation of the number of molecules in a library is the most critical step in the preparation steps for next-generation sequencing (NGS) to obtain high-quality reading data. Indeed, in order to obtain reliable and quality reading data, it is necessary to use a sufficient quantity of DNA, and in particular for Illumina® sequencing. The use of insufficient DNA will result in low sequencing efficiency and DNA overabundance will increase cluster density and result in poor quality data.
Quantitative PCR is considered to be the most accurate and efficient method of quantification of NGS library.
The library quantitation kits for Illumina® sequencing contain all the components necessary for the qPCR quantification of libraries:
- Mix qPCR containing DNA polymerase, dNTPs and buffer
- Primers to amplify only the molecules that contain the two sequences of adapters
- The DNA standards (S1 to S6)
- A ROX solution to add depending on the device used. If the ROX solution is separate from the mix, it is a universal kit that can be used with all instruments.