TET activity assay kits are research tools developed to quantify the enzymatic activity of Ten-Eleven Translocation (TET) proteins by measuring the hydroxylation of 5-methylcytosine (5mC) into oxidized derivatives such as 5-hydroxymethylcytosine (5hmC). These assays provide a reliable approach for studying DNA demethylation mechanisms and epigenetic regulation.

Assay Features

Most kits measure the activity of TET1, TET2, and TET3 using purified proteins or nuclear extracts. Depending on the platform, detection is fluorometric, colorimetric, or chemiluminescent, with assay completion typically achieved within 4–5 hours using a standard microplate reader.

Applications

• Quantification of endogenous TET enzyme activity in cells and tissues.
• Evaluation of TET inhibitors or activators in epigenetic studies.
• Comparison of TET isoform activity under different experimental conditions.
• Research in epigenetics, cancer biology, stem cell biology, and developmental biology.