G-Series Mouse Cytokine Array 4000 Kit. A combination of 5 non-overlapping arrays to measure the relative expression levels of 200 mouse cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 16 Sample Kit, 32 Sample Kit, 64 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 200 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
Product Features
- High sensitivity (pg/mL to ng/mL levels)
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Test 16 samples on each slide
- Data analysis software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137)
| 6Ckine (CCL21)
| Ace
| Activin A
| ADAMTS-1 (METH1)
|
| Adiponectin (ACRP30)
| ALK-1
| Amphiregulin
| ANGPTL1
| ANGPTL3
|
| Artemin
| Axl
| BAFF R
| Betacellulin (BTC)
| bFGF
|
| BLC (CXCL13)
| C5a
| Cardiotrophin-1 (CT-1)
| CCL28 (MEC)
| CCL6
|
| CD27 (TNFRSF7)
| CD27 Ligand (TNFSF7)
| CD30 (TNFRSF8)
| CD30 Ligand (TNFSF8)
| CD32a
|
| CD36 (SR-B3)
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
| CD48 (SLAMF2)
| CD6
|
| CD80 (B7-1)
| Chemerin
| Chordin
| Clusterin
| CRP (C-Reactive Protein)
|
| CTLA-4 (CD152)
| CXCL16
| Cystatin C
| DAN
| Decorin
|
| DKK-1
| DLL4
| Dtk
| E-Cadherin
| EDAR
|
| EGF
| Endocan
| Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
| Epigen
| Epiregulin
| E-Selectin
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
| Fetuin A
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| Galectin-1
| Galectin-3
|
| Galectin-7
| Gas 1
| Gas 6
| GCSF
| GITR (TNFRSF18)
|
| GITR Ligand (TNFSF18)
| GM-CSF
| gp130
| Granzyme B
| Gremlin-1
|
| H60
| HAI-1
| HGF
| HGFR
| I-309 (TCA-3/CCL1)
|
Species Detected
Mouse
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Detection of Relative Protein Expression
- Detecting Patterns of Cytokine Expression
- Biomarker Screening
- Identifying Key Factors
- Confirming a Biological Process
Kit Components
- Antibody Array Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-4.gal)
GAL (GAL-QAM-CYT-5.gal)
GAL (GAL-QAM-CYT-6.gal)
GAL (GAL-QAM-CYT-7.gal)
GAL (GAL-QAM-CYT-8.gal)
Excel (GSM-CYT-4_Analysis_Tool.xlsx)
Excel (GSM-CYT-5_Analysis_Tool.xlsx)
Excel (GSM-CYT-6_Analysis_Tool.xlsx)
Excel (GSM-CYT-7_Analysis_Tool.xlsx)
Excel (GSM-CYT-8_Analysis_Tool.xlsx)
citations
Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Skin samples in psoriasis model)
- Year:
- 2020
- Journal Name:
- Immunity
- PubMed ID:
- 32553276
Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (brain tissue lysates from AD and normal patients)
- Year:
- 2019
- Journal Name:
- Cell Research
Fate mapping RNA-sequencing reveal Malat1 regulates Sca1+ progenitor cells to vascular smooth muscle cells transition in vascular remodeling
- Species:
- Mouse
- Sample Type:
- Sca1+ stem/progenitor cells (SPCs)
- Year:
- 2023
- Journal Name:
- Cellular and Molecular Life Sciences
Inducing the “re-development state” of periodontal ligament cells via tuning macrophage mediated immune microenvironment
- Species:
- Mouse
- Sample Type:
- RAW264.7 cell
- Year:
- 2023
- Journal Name:
- Journal of advanced research
Injury-induced interleukin-1 alpha promotes Lgr5 hair follicle stem cells de novo regeneration and proliferation via regulating regenerative microenvironment in mice
- Species:
- Mouse
- Sample Type:
- Dorsal skin tissues
- Year:
- 2023
- Journal Name:
- Inflammation and regeneration
Progesterone prevents HGSOC by promoting precancerous cell pyroptosis via inducing fibroblast paracrine
- Species:
- Mouse
- Sample Type:
- ascites
- Year:
- 2023
- Journal Name:
- iScience
Single-Cell Sequencing Informs That Mesenchymal Stem Cell Alleviates Renal Injury Through Regulating Kidney Regional Immunity in Lupus Nephritis
- Species:
- Mouse
- Sample Type:
- kidney tissue
- Year:
- 2023
- Journal Name:
- Stem Cells and Development
Correction: A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner
- Species:
- Human
- Sample Type:
- THP-1 cell, Human Dermal Lymphatic Endothelial Cells (HDLEC)
- Year:
- 2022
- Journal Name:
- Angiogenesis
ORFV infection enhances CXCL16 secretion and causes oncolysis of lung cancer cells through immunogenic apoptosis
- Species:
- Mouse
- Sample Type:
- LLC cell-xenograft, A549 cell-xenograft
- Year:
- 2022
- Journal Name:
- Frontiers in cellular and infection microbiology
Minmin Jiang, Wenying Cai, Junmin Zhang, Ting Xie, Liyan Xi, Xiqing Li, Jiufeng Sun, Melanization of a meristematic mutant of Fonsecaea monophora increase the pathogenesis in a BALB/c mice infection model, Medical Mycology, Volume 56, Issue 8, November 2018, Pages 979-986, https://doi.org/10.1093/mmy/myx148
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2018
- Journal Name:
- Medical Mycology


