Dolichos biflorus (DBA) (AP)
Size : 1mg
Brand : US Biological
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze
Alkaline phosphatase (AP) is conjugated to Dolichos biflorus Lectin (DBA) to show the binding of DBA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140kD) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.
A1 >> A2
Ca++, Mn++, Mg++, Zn++
Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Alkaline Phophatase (AP).
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.