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> Recombinant SARS-CoV-2 S1 Subunit Protein (RBD) with C-terminal Mouse IgG Fc Tag

Recombinant SARS-CoV-2 S1 Subunit Protein (RBD) with C-terminal Mouse IgG Fc Tag

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Referentie 230-30166-10

Formaat : 10ug

Merk : RayBiotech

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Recombinant SARS-CoV-2 S1 Subunit Protein (RBD) with C-terminal Mouse IgG Fc Tag

Recombinant SARS-CoV-2 S1 subunit protein (RBD) with C-terminal mouse IgG Fc-tag, derived from the transfected human HEK293 cells.
Datasheet

Product Description

Specifications

Size 10 ug, 50 ug
Species SARS-CoV-2
Accession Number QHD43416
Protein Name / Synonyms Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Expressed Region Arg319-Phe541
Expression System HEK293 cells
Tag C-terminal mouse IgG Fc-tag
Purity >95 %
Purity Determined By SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa) Recombinant protein product has a calculated molecular mass of 50 kDa including 25 kDa mouse IgG Fc-tag. Due to the abundant glycosylation, it migrates as approximately ∼65 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Format Liquid
Formulation Supplied as a 0.2 µm filtered solution in PBS (pH 7.4)
Concentration (lot specific) Lot specific (see the label on the vial), determined by BCA protein assay
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C

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SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with deglycosylation under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size (˜50 kDa), thus indicating that the untreated recombinant protein (Lane 2, ˜65 kDa) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein (˜65 kDa, red arrow) under reducing conditions.
Lane 3: Treated protein (˜50 kDa, green arrow) with deglycosylation enzymes under native conditions.
Lane 4: Treated protein (˜50 kDa, green arrow) with deglycosylation enzymes under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA. Recombinant S1/RBD-mFc (Code: 230-30166) can bind immobilized recombinant human ACE2 (Code: 230-30165) with EC50 at 3.2 ng/mL.

Stability & Storage

Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

Spike Protein, S1 Subunit
Spike Protein, S2 Subunit
Nucleocapsid Protein
S1 Subunit Protein (RBD) with C-terminal Fc tag
S1 Subunit Protein (RBD) with C-terminal His-tag
S1 Subunit Protein with C-terminal His-tag
S2 Subunit Protein with C-terminal His-tag
Nucleocapsid Protein with C-terminal His-tag

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). .

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