Product Description

ERBB2/CEN 17 CISH Probe Kit is designed for the qualitative detection of amplifications involving the human ERBB2 gene as well as the detection of chromosome 17 alpha satellites in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

Form

Liquid

Recommend Usage

20x Wash Buffer TBS (WB5) is to be prepared according to the instructions in Assay procedure. All other kit reagents are ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.

Supplied Product

Reagent Provided:

This Probe is composed of:
1. Digoxigenin-labeled polynucleotides, which target sequences mapping in 17q12 (chr17:37,725,661-37,973,541) harboring the ERBB2 gene region.
2.Dinitrophenyl-labeled polynucleotides, which target sequences mapping in 17p11.1-q11.1 specific for the alpha satellite centromeric region D17Z1 of chromosome 17.
3. Formamide based hybridization buffer.

Probe Position

Regulatory Status

For research use only (RUO)

Storage Instruction

Store at 2-8°C in an upright position. Return to storage conditions immediately after use.

Note

Interpretation of the results must be made within the context of the patient´s clinical history with respect to further clinical and pathologic data of the patient by a qualified pathologist.

Interpretation of Result